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Integrated mass spectrometric and multivariate data analysis approaches for the discrimination of organic and conventional strawberry (Fragaria ananassa Duch.) crops

TitleIntegrated mass spectrometric and multivariate data analysis approaches for the discrimination of organic and conventional strawberry (Fragaria ananassa Duch.) crops
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2015
AuthorsD'Urso, G., d'Aquino Luigi, Pizza C., and Montoro P.
JournalFood Research International
Volume77
Pagination264-272
ISSN09639969
Keywords(HR)-LC-MS, Anthocyanins, Chromatographic analysis, Crops, Discriminant analysis, Flavonoids, Fragaria ananassa, Fruits, Least squares approximations, liquid chromatography, Mass spectrometers, Mass Spectrometry, Metabolic profiling, metabolism, Metabolites, metabolomics, Multivariant analysis, Phenols, Principal component analysis, Spectrometry, Strawberry
Abstract

In the present study, metabolic changes in secondary metabolites of Fragaria ananassa Duch. fruits during ripening were evaluated, and in particular, the differences between fruits obtained from either organic or conventional, non-organic crops. Extracts from ripe and unripe fruits of Fragaria ananassa var. Candonga were analyzed by direct infusion mass spectrometry using an instrument equipped with an ion trap analyzer (DI-ESI-IT-MS) and by liquid chromatography coupled to high resolution mass spectrometry using a mass spectrometer equipped with an ESI source and an Orbitrap analyzer (LC-ESI-FT-MS). Chromatograms were evaluated using the free software package MZmine and the resulting metabolomics data were processed. Data sets were analyzed by Principal Component Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS-DA). All the differences between the fruits collected from organic crops and conventional crops were underlined. In addition, LC-ESI-FT-MS analyses were useful to achieve a comprehensive characterization of metabolic profiles of strawberries (flavonoids, anthocyanins and catechins were identified in the extracts). Flavonoids and anthocyanins were present with similar metabolic profile in F. ananassa obtained from organic and conventional, non-organic crops with differences from ripe and unripe fruits. Phenolic compounds thus are not responsive for the classification observed by untargeted metabolomic approach. © 2015 Elsevier Ltd.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84947017034&doi=10.1016%2fj.foodres.2015.04.028&partnerID=40&md5=b24f5c2c9a5417b8588282d111ca8148
DOI10.1016/j.foodres.2015.04.028
Citation KeyD'Urso2015264