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Regulation of cytokine production in aging: Use of recombinant cytokines to upregulate mitogen-stimulated spleen cells

TitleRegulation of cytokine production in aging: Use of recombinant cytokines to upregulate mitogen-stimulated spleen cells
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication1997
AuthorsFrasca, D., Pucci S., Goso C., Barattini P., Barile S., Pioli Claudio, and Doria G.
JournalMechanisms of Ageing and Development
Volume93
Pagination157-169
ISSN00476374
KeywordsAging, animal cell, animal experiment, Animals, CD4 antigen, CD4-Positive T-Lymphocytes, Cells, conference paper, Cultured, cytokine, Cytokines, Developmental, Female, gamma interferon, gene expression regulation, Genetic, helper cell, immunoregulation, Inbred C57BL, Interferon Type II, interleukin 2, interleukin 4, Interleukin-2, Interleukin-4, lymphocyte activation, male, Messenger, messenger RNA, Mice, mouse, nonhuman, priority journal, Recombinant Proteins, RNA, Spleen, spleen cell, T-Lymphocytes, Th1 Cells, Th2 Cells, Transcription
Abstract

We investigated the production of IL-2 and IFN-γ (Th1 type) and IL-4 (Th2 type) cytokines by mitogen-activated spleen cells from young, adult and old mice. Cytokine production was evaluated in culture supernatants by CTLL proliferation (IL-2), ELISA (IFN-γ), CT4.S proliferation (IL-4) and in mRNA extracted from activated CD4 + cells by RT-PCR (IL-2, IFN-γ and IL-4). Results show that the production of IL-2, as protein and mRNA, is profoundly depressed by aging, whereas that of IFN-γ, as protein and mRNA, firstly declines and then increases with age. The production of IL-4, as protein, monotonically declines with aging whereas, as mRNA, firstly decreases and then increases above the level in young mice. Spleen cells in culture were also incubated with mitogens and with a recombinant cytokine (IL-1β, IL-2, IL-3, IL-4, IL-12 or IFN-γ) at various concentrations. It was found that recombinant cytokines by and large enhance cytokine production when the level induced by mitogens only is low. This conclusion applies to IL-2 and IFN-γ production as protein and mRNA. The addition of recombinant cytokines also increases the production of IL-4 at the protein level in spleen cells from old mice but, at the mRNA level, only in spleen cells from young mice. This finding suggests age-related changes in IL-4-specific mRNA transcription rate and post-transcriptional half-life as well as translation kinetics.

Notes

cited By 30

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0030970254&doi=10.1016%2fS0047-6374%2896%2901825-8&partnerID=40&md5=ebf9d40ae56798b2486182697e71a496
DOI10.1016/S0047-6374(96)01825-8
Citation KeyFrasca1997157