Sorry, you need to enable JavaScript to visit this website.

Description of the bacterial rna polymerase inhibitor ge23077-producer actinomadura sp. Nrrl b-65521t as actinomadura lepetitiana sp. nov.

TitoloDescription of the bacterial rna polymerase inhibitor ge23077-producer actinomadura sp. Nrrl b-65521t as actinomadura lepetitiana sp. nov.
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2020
AutoriDalmastri, Claudia, Gastaldo L., Berini F., Marinelli F., and Marcone G.L.
RivistaInternational Journal of Systematic and Evolutionary Microbiology
Volume70
Paginazione4782-4790
ISSN14665026
Abstract

The filamentous actinomycete that produces the antibiotic GE23077 was isolated by the Lepetit Research Group from a soil sample collected in Thailand, and it was classified as a member of the genus Actinomadura on the basis of its morphol-ogy and cell-wall composition. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain formed a distinct monophyletic line within the genus Actinomadura, and it was most closely related to Actinomadura bangladeshensis DSM 45347T (99.31% similarity) and Actinomadura mexicana DSM 44485T (98.94%). The GE23077-producing strain formed an extensively branched, non-fragmented vegetative mycelium; no pseudosporangia were formed and the arthrospores were organized in slightly twisted chains. The cell wall contained meso-2,6-diaminopimelic acid and the diagnostic sugar was madu-rose. The predominant menaquinone was MK-9(H6), with minor amounts of MK-9(H8) and MK-9(H4). The diagnostic phospho-lipids were phosphatidylinositol and diphosphatidylglycerol. The major cellular fatty acids were C16:0 and tuberculostearic acid (10-methyloctadecanoic acid), followed by minor amounts of C18:1ω9c, C16:1ω7c and 10-methylheptadecanoic acid. The genomic DNA G+C content was 71.77 mol%. Significant differences in the morphological, chemotaxonomic and biochemical data, and the low DNA–DNA relatedness between the GE23077-producing strain and closely related type strains clearly demonstrate that it represents a novel species of the genus Actinomadura, for which the name Actinomadura lepetitiana sp. nov. is proposed. The type strain is NRRL B-65521T(=LMG 31258T=DSM 109019T). © 2020 The Authors.

Note

cited By 0

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85090069211&doi=10.1099%2fijsem.0.004348&partnerID=40&md5=c6b0636d59969cf3df9fec8642744aef
DOI10.1099/ijsem.0.004348
Citation KeyDalmastri20204782